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Image Search Results
Journal: Stem cell research
Article Title: Non-invasively enhanced intracranial transplantation of mesenchymal stem cells using focused ultrasound mediated by overexpression of cell-adhesion molecules.
doi: 10.1016/j.scr.2020.101726
Figure Lengend Snippet: Fig. 3. CAM co-localization. Co-localization of ICAM-1-positive cells and astrocytes (A), endothelial cells (B), microglia (C) and VCAM-1 cells and endothelial cells (D). Scale bar, 200 μm.
Article Snippet: Sections were blocked with 5% normal goat serum (Vector Labs, Burlingame, CA, USA) and incubated with primary antibodies at the following dilutions: ICAM-1 (sc-8439; Alexa Fluor 488; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA), VCAM-1 (sc-13,160; Alexa Fluor 647; 1:100; Santa Cruz Biotechnology), microglia (Iba1; 019–19,747; 1:300; Wako Chemicals, Richmond, VA, USA), astrocytes (glial fibrillary acidic protein; ab116010; 1:300; Abcam, Cambridge, UK), and
Techniques:
Journal: PLoS ONE
Article Title: Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats
doi: 10.1371/journal.pone.0098639
Figure Lengend Snippet: (A) From left to right: markers of cells that make up the blood brain barrier (a, e, i, m; green), Ang1 (b, f, j, n; red), 4′,6-diamidino-2-phenylindole (DAPI) stain (c, g, k, o; blue), and a merged image (d, h, l, p). RECA1 is an endothelial cell marker protein; PDGFRβ is a pericyte marker; GFAP is an astrocyte marker; and MAP2 is a neuronal cell marker. RECA1, rat endothelial cell antigen; PDGFRβ, platelet-derived growth factor receptor β; GFAP, glial fibrillary acidic protein; MAP2, microtubule-associated protein 2; Ang1, angiopoietin-1. The scale bars are 10 µm. (B) Three-dimensional (3D) images of endogenous Ang1 in non-ischemic brains visualized with a confocal laser microscope. a: endothelial cell marker RECA1 (green) and endogenous Ang1 (red); b: neuronal cell marker MAP2 (green) and endogenous Ang1 (red). RECA1, rat endothelial cell antigen; Ang1, angiopoietin-1; MAP2, microtubule-associated protein 2. An arrow indicates Ang1 expressed in a pericyte. Arrowheads indicate Ang1 expressed in neuronal cells.
Article Snippet: The primary antibodies that were used were a
Techniques: Staining, Marker, Derivative Assay, Microscopy
Journal: PLoS ONE
Article Title: Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats
doi: 10.1371/journal.pone.0098639
Figure Lengend Snippet: (A) Western blot analysis with an anti-Ang1 antibody. Each group N = 5. tPA, tissue plasminogen activator; PMCAO, permanent middle cerebral artery occlusion; Ang1, angiopoietin-1. (B) Measurements with a densitometer.
Article Snippet: The primary antibodies that were used were a
Techniques: Western Blot
Journal: PLoS ONE
Article Title: Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats
doi: 10.1371/journal.pone.0098639
Figure Lengend Snippet: (A) Immunohistochemical staining with an anti-Ang1 antibody. Representative findings are shown of the peri-infarct and infarct areas of the control, tPA-1h, tPA-4h, and PMCAO groups. High magnification (1,000×) is shown in the upper right of the low magnification (200×) photograph. Ang1-positive vessels were shown by asterisk. tPA, tissue plasminogen activator; PMCAO, permanent middle cerebral artery occlusion; Ang1, angiopoietin-1. The black scale bar is 10 µm, and the red scale bar is 100 µm. (B) Mean number of Ang1-positive vessels. Three locations were chosen randomly in the control cerebral cortex and in each infarct area and peri-infarct area. The figures are the mean number of Ang1-positive vessels from 3 random fields of view of an optical microscope at 200× magnification. Each group N = 5.
Article Snippet: The primary antibodies that were used were a
Techniques: Immunohistochemical staining, Staining, Microscopy
Journal: PLoS ONE
Article Title: Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats
doi: 10.1371/journal.pone.0098639
Figure Lengend Snippet: From left to right: markers of cells that make up the blood brain barrier (a, e, i; red), neuronal cell (m; red), COMP-Ang1 protein (b, f, j, n; green), DAPI stain (c, g, k, o; blue), and a merged image (d, h, l, p). Immunostaining with an anti-FLAG antibody was performed on COMP-Ang1 proteins in order to differentiate them from endogenous Ang1. RECA1 is an endothelial cell marker protein; PDGFRβ is a pericyte marker; GFAP is an astrocyte marker; and MAP2 is a neuronal cell marker. RECA1, rat endothelial cell antigen; PDGFRβ, platelet-derived growth factor receptor; GFAP, glial fibrillary acidic protein; Ang1, angiopoietin-1. The scale bars are 10 µm.
Article Snippet: The primary antibodies that were used were a
Techniques: Staining, Immunostaining, Marker, Derivative Assay
Journal: PLoS ONE
Article Title: Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats
doi: 10.1371/journal.pone.0098639
Figure Lengend Snippet: These panels show the amount of cerebral hemorrhage (A), cerebral edema volume (B), cerebral infarct volume (C), and the prognosis with a 6-point neurological scale score (D) 24 h after ischemia. A-C were performed on the COMP-Ang1 group (N = 5) and the COMP group (N = 5). D was performed on the COMP-Ang1 group (N = 11) and the COMP group (N = 9). Cerebral edema and infarct volumes are expressed as proportions on the ischemic side of the cerebral hemisphere. The amount of cerebral hemorrhage is expressed as hemoglobin concentration in a whole cerebral homogenate. COMP; cartilage oligomeric protein, COMP-Ang1; cartilage oligomeric protein-angiopoietin-1.
Article Snippet: The primary antibodies that were used were a
Techniques: Concentration Assay